
Terreplenish® Case Study :
Congo Crop Trials
ASAGEELS
DEPARTMENT OF AGRICULTURE

Testing Terreplenish Fertilizer on Congolese Soil:
The Case of the Kimpoko Site in Kinshasa
Conducted by: Donatien Mudimba Ilunga, Agronomist and Plant
Scientist:
Zelia Tshiala Ntata, Agronomist in Natural Resource Management (Soil and Water)
Introduction:
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In a global context marked by climate change, the scarcity of natural resources, and the increasing degradation of soils, agriculture faces a dual challenge: feeding a constantly growing population while preserving the sustainability of ecosystems. This challenge is even more pressing in developing countries, where agricultural systems are often weakened by poor soil quality, limited access to quality inputs, and greater exposure to climatic hazards such as drought, erosion, or the depletion of organic matter (FAO, 2015; IPCC, 2022; UNEP, 2016).
In these regions, long-term dependence on chemical fertilizers has often worsened the biological imbalances of the soil, leading to a progressive decline in fertility, a loss of microbial biodiversity, and structural degradation that compromise medium- and long-term productivity (Lal, 2020). It is in this context that the use of biological and soil-regenerating fertilizers, such as Terreplenish, is gaining increasing interest. Developed through the controlled fermentation of organic residues, this natural liquid fertilizer is rich in beneficial microorganisms, organic acids, and essential nutrients. Its purpose is to restore the soil’s microbiological balance, improve its structure, enhance its water-retention capacity, and optimize the absorption of nutrients by plants (Terreplenish®, n.d.).
In developing countries, where agriculture is the main source of livelihood for a large part of the population, the introduction of environmentally viable, low-cost solutions adapted to local conditions is a priority (UNEP, 2016; Lal, 2020). The trial of Terreplenish therefore falls within an effort to seek sustainable solutions aimed at improving agricultural productivity while preserving natural balances.
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Overall Objective
To evaluate the agronomic effectiveness of the biological fertilizer Terreplenish on crop growth and development in the agroecological context of Kinshasa.
Specific Objectives
Compare the growth and yield performance of a control crop with and without the application of Terreplenish. Test the adaptability of the fertilizer: verify whether the fertilizer performs well under the specific conditions of the field (soil type, climate, crop). Optimize the use of the fertilizer: adjust the dosage and frequency of application to achieve the best results. Assess improvements in soil fertility: observe whether the fertilizer enriches the soil with essential nutrients (nitrogen, phosphorus, potassium, etc.).
Research Questions
What differences can be observed between the yields of crops treated with Terreplenish and those of the untreated control? Is the Terreplenish fertilizer effective under the specific conditions of the Kimpoko site (soil type, climate, and tested crops)? What is the optimal dose and frequency of Terreplenish application to maximize the growth and yield of vegetable crops? Does the use of Terreplenish improve the soil content of essential nutrients such as nitrogen (N), phosphorus (P), and potassium (K)?
Hypotheses
The yields of crops treated with Terreplenish will be higher than those of untreated control crops. Terreplenish fertilizer will effectively adapt to the specific conditions of the Kimpoko site, improving crop growth despite local constraints (soil, climate, crop type). An appropriate dosage and application frequency of Terreplenish will allow for optimal vegetative development and higher crop yields. The application of Terreplenish will significantly increase the soil content of essential nutrients, particularly nitrogen, phosphorus, and potassium, compared to untreated soils.
MATERIALS AND METHODS
1. Materials Used In this study conducted in Kinshasa, Democratic Republic of Congo, several materials were used to evaluate the effectiveness of the organic fertilizer Terreplenish on soil quality and the growth of certain vegetable crops. The main materials used were as follows:
A. Terreplenish Fertilizer A natural liquid fertilizer, derived from the controlled fermentation of organic matter, rich in beneficial microorganisms, organic acids, and essential nutrients. It was used as the main treatment in the various experimental plots.
B. Vegetable Seeds Four types of crops were selected due to their economic and nutritional importance in the study region:
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Amaranth (Amaranthus spp.), a nutrient-rich leafy plant.
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Spinach (Spinacia oleracea), a short-cycle crop valued for its edible leaves
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Okra (or gombo, Abelmoschus esculentus), a popular fruit vegetable in the local diet.
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Cucumber (Cucumis sativus), commonly grown in urban and peri-urban areas.
These crops allowed us to compare the effect of fertilizer on different types of plants: leaves and fruits.
2. Methodology This study was conducted to evaluate the effects of the organic fertilizer Terreplenish on the growth and development of four vegetable crops (amaranth, spinach, okra, and cucumber) in Kinshasa, Democratic Republic of Congo, using an open-field experimental design comprising two main treatments:
T1: Soil with application of Terreplenish
T0: Control without fertilizer or with conventional chemical fertilizer.
2.1. Preparation of experimental plots
Six (6) plots measuring 1 meter by 7 meters (1.20 m × 15 m) were manually demarcated and prepared. Before any intervention, each plot was amended with poultry manure to improve the soil's organic matter content.

Figure 1 : Field delineation and plot formation
2.2. Treatment allocation The six plots were allocated to crops and treatments as follows:
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Plot 1 (T1): Cucumber treated with Terreplenish
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Plot 2 (T1): Okra (okra) treated with Terreplenish
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Plot 3 (T0): Okra + cucumber control (without Terreplenish)
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Plot 4 (T0): Spinach + amaranth control (without Terreplenish)
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Plot 5 (T1): Amaranth treated with Terreplenish
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Plot 6 (T1): Spinach treated with Terreplenish This allocation allows for a direct comparison between crops treated with Terreplenish and those grown without the product (controls).
2.3. Initial Application of Terreplenish
Terreplenish fertilizer was applied to the soil on designated plots before sowing, at a rate of 500 ml diluted in 12 liters of water on four plots previously treated with Terreplenish, as a biological amendment. A period of 7 days was allowed between application and sowing to allow for decomposition and microbial activation of the product in the soil.

Figure 2: Application of Terreplenish in the near-planting area
2.4. Sowing the crops
The seeds of the four crops (amaranth, spinach, ngombo, and cucumber) were sown manually after 7 days, according to the spacing and densities recommended for each species.

Figure 3: Crop Sowing
2.5. Foliar Application of Terreplenish
After crop emergence, Terreplenish was applied as a foliar spray every 7 days, at a rate of 500 ml diluted in 12 liters of water, to all four plots treated with Terreplenish. This regular application was intended to enhance vegetative growth and plant vigor in the treated plots.
2.6. Measured Parameters
In this trial, the effectiveness of the Terreplenish fertilizer was evaluated exclusively through agronomic indicators related to final crop yield. Observations focused on plant behavior from germination to harvest. The following is a detailed description of the parameters analyzed:
1. Number of days to germination
➤ This is the number of days between sowing and the emergence of the first seedlings. This parameter allows us to assess how quickly the crop starts, a good indicator of initial seed vitality and soil quality.
2. Germination rate
➤ This rate corresponds to the percentage of seeds that have successfully germinated compared to the total number sown. A high rate reflects favorable soil conditions for emergence, as well as good seed vigor.
3. Emergence percentage
➤ Similar to the germination rate, this parameter specifies the number of seedlings that have emerged from the soil surface. It takes into account environmental conditions (humidity, temperature, soil compaction) that influence emergence.
4. Crop development
➤ This criterion combines the observation of plant growth over time (height, foliage, density, general appearance). It allows you to judge the vigor and the proper progress of the vegetative phases.
5. Resistance to Fungi and Insects
➤ This parameter assesses the plants' tolerance to biotic attacks (fungal diseases, insect pests). Better resistance may indicate a well-nourished plant and biologically active soil.
6. Start of Harvest after Sowing
➤ This time frame expresses the time between sowing (or transplanting) and crop maturity. It provides an indication of the earliness induced by fertilizer.
7. Quantity Harvested in Direct Seeding
➤ This parameter measures productivity in direct cultivation (without prior plowing or mechanical tillage). It allows us to estimate the impact of Terreplenish in systems with low soil disturbance.
8. Crop Cycle (from Transplanting to Harvest)
➤ This cycle corresponds to the total development time of a transplanted crop, measured between transplanting and harvest. It is an indicator of the speed of growth and maturity.
9. Yield per hectare
➤ This is the primary agronomic performance criterion, expressing the total quantity of production (kg/ha or t/ha). This parameter allows for a direct comparison of the effects of fertilizer on productivity.
RESULTS
1. Germination

Figure 4: Germination of crops
2. First post-emergence treatment
PLOT TREATED WITH FERTILIZER
CONTROL PLOT WITHOUT FERTILIZER

Figure 5: First treatment on okra
Early and vigorous growth in the plot treated with Terreplenish fertilizer, compared to the untreated plot where development was slower, a sign of stress or less favorable conditions.
PLOT TREATED WITH FERTILIZER
CONTROL PLOT WITHOUT FERTILIZER

Figure 6: Amaranth crops
The difference is clear: the treated plants have larger leaves, denser foliage, and a deeper green color. The untreated plants appear stunted, suggesting less effective nutrition.
PLOT TREATED WITH FERTILIZER
CONTROL PLOT WITHOUT FERTILIZER

Figure 7: Spinach crops
Treated spinach exhibited faster growth and better leaf coverage. Control spinach exhibited less uniform development, demonstrating the positive influence of Terreplenish on vegetative vigor
PLOT TREATED WITH FERTILIZER
CONTROL PLOT WITHOUT FERTILIZER

The treated plants are more developed and exhibit more pronounced vertical growth, accompanied by more robust stems. The controls show visible growth retardation.
3. Second post-emergence treatment
PLOT TREATED WITH FERTILIZER
CONTROL PLOT WITHOUT FERTILIZER

Figure 8 cucumber crops

Figure 9: 15-day amaranths

Figure 10: Spinach and okra crops
Growth stimulation is visible. Treated plants display greater density and height. Control plants show leaf loss and signs of deficiency, indicating limited nutritional intake
4. Third post-emergence treatment

Figure 11: 3 Week Cucumbers

Figure 12: 21-day-old amaranths

Figure 13: 3-week-old okra33

Figure 14: Spinach
5. Fourth post-emergence treatment

Figure 15: 30-day-old cucumbers

Figure 16: 30-day spinach

Figure 17: Okra in the fourth post-emergence treatment
6. Fifth post-emergence treatment

Figure 18: 40-day-old cucumbers

Figure 19: 40-day-old okra
The treated plot shows healthier plants and better overall development, suggesting a positive effect of the treatment, while the untreated plot shows slower and less uniform growth.
7. ANNEX CROPS
CASSAVA LEAF CROPS
• First treatment
CONTROL PLOT WITHOUT FERTILIZER
TREATED WITH FERTILIZER

Large leaves and rapid growth for treated cassava leaves, whereas growth is normal for untreated cassava leaves.
• Second treatment
TREATED WITH FERTILIZER
CONTROL PLOT WITHOUT FERTILIZER

• Third treatment
TREATED WITH FERTILIZER
CONTROL PLOT WITHOUT FERTILIZER

• Fourth treatment
TREATED WITH FERTILIZER
CONTROL PLOT WITHOUT FERTILIZER

Cassava leaves treated with Terreplenish can be harvested every two weeks, but not every three weeks.
8. Harvesting


Figure 20: Harvesting Crop Products
Yields appear significantly higher in treated plots. Harvested crops are of better size and appearance, demonstrating the overall effectiveness of the biological treatment on final production.
9. Comparative Evaluation of Crops with and without Terreplenish Treatment

Table 1: Comparative Evaluation of Crops with and without Fertilizer Treatment
10. Interpretation of Results
The results obtained from this experiment show that Terreplenish is a high-quality fertilizer, both agronomically and environmentally. Indeed, the crops treated with Terreplenish exhibited faster growth, more uniform vegetative development, and greater resistance to environmental stresses such as drought or temperature fluctuations. These observations indicate a significant improvement in plant health, directly linked to the supply of nutrients and beneficial microorganisms contained in the fertilizer.
Furthermore, Terreplenish contributes to improving the structure and biological fertility of the soil. It stimulates microbial activity, thereby promoting the decomposition of organic matter and the gradual release of essential nutrients (nitrogen, phosphorus, potassium). This action helps maintain a living and balanced soil, which is fundamental for the sustainability of agricultural systems.
In comparison, the untreated control plots showed slower growth, lower yields, and reduced plant vitality, highlighting the product’s effectiveness. In addition, since Terreplenish is a biological fertilizer, it does not pose the pollution risks associated with chemical fertilizers, such as groundwater contamination or the degradation of soil biodiversity.
In the context of climate change, where farmers must cope with increasingly unpredictable growing conditions, the use of biological products such as Terreplenish becomes a sustainable and responsible solution. It helps strengthen the resilience of agricultural systems while respecting the environment, making it a relevant choice for more sustainable agriculture.
CONCLUSION
In conclusion, the multiple benefits observed during this experiment demonstrate the remarkable effectiveness of Terreplenish fertilizer on crop growth, improved soil fertility, and natural plant resistance. Its rapid action, long-lasting effect, and positive impact even in unfavorable soils make it a solution adapted to the agricultural realities of tropical environments. Given these results, Terreplenish is highly recommended to all farmers in Kinshasa and throughout the Democratic Republic of Congo as an effective, sustainable, and environmentally friendly organic fertilizer.
BIBLIOGRAPHY
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1. FAO. (2015). Status of the World’s Soil Resources (SWSR) – Main Report. Food and Agriculture Organization of the United Nations. https://www.fao.org/3/i5199e/i5199e.pdf
2. IPCC (IPCC). (2022). Climate Change 2022: Impacts, Adaptation and Vulnerability. Intergovernmental Panel on Climate Change.
3. UNEP. (2016). Unlocking the Sustainable Potential of Land Resources: Evaluation Systems, Strategies and Tools. United Nations Environment Program.
4. Terreplenish®. (n.d.). Product Overview and Agronomic Benefits. Official company website or product data sheet. https://www.terreplenish.com
5. Lal, R. (2020). Regenerative agriculture for food and climate. Journal of Soil and Water Conservation, 75(5), 123A-124A.